What question did this study set out to answer?

To assess the elicitor activity, structural requirements, and disease-resistance capabilities of laminarin and related beta-glucans in tobacco cells and leaves.

November 1, 2000PLANT PHYSIOLOGYOpen Access

Linear β-1,3 Glucans Are Elicitors of Defense Responses in Tobacco

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Authors

OKO. KlarzynskiGoodyear (United Kingdom)BPBertrand PlesseCentre National de la Recherche Scientifique JJJean-Marie JoubertSaint-Gobain (France)

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Implication

Experimental study demonstrates laminarin activates systemic defenses and reduces bacterial infection in tobacco, indicating the immune-eliciting capacity of linear beta-1,3 glucans.

Key Points

To assess the elicitor activity, structural requirements, and disease-resistance capabilities of laminarin and related beta-glucans in tobacco cells and leaves.
Extracted and purified laminarin from Laminaria digitata and compared its elicitor activity against oligogalacturonides in suspension-cultured Nicotiana tabacum cells.
Evaluated extracellular alkalinization, hydrogen peroxide release, defense enzyme activities, salicylic acid accumulation, and structure-activity relationships using various glucan oligomers.
Infiltrated tobacco leaves with 200 µg/mL laminarin, assayed pathogenesis-related proteins at 48 hours, and challenged leaves with Erwinia carotovora subsp. carotovora 5 days post-treatment.
Treatment with 200 µg/mL laminarin triggered a 1.9-pH-unit extracellular alkalinization, transient H2O2 production, strong activation of PAL, COMT, and LOX, and salicylic acid accumulation without causing cell death.
Structure-activity analysis demonstrated that defense elicitation in tobacco is specifically dependent on linear beta-1,3 linkages, identifying laminaripentaose as the minimal active structure.
Infiltration of laminarin into leaves stimulated four families of antimicrobial pathogenesis-related proteins within 48 hours and caused a strong reduction in bacterial soft rot infection after pathogen challenge.

Cite This Study

Klarzynski et al. (2000) studied this question.

synapsesocial.com/papers/6a9e1c44c448d60cdc367fee https://doi.org/10.1104/pp.124.3.1027

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Implication

Experimental study demonstrates laminarin activates systemic defenses and reduces bacterial infection in tobacco, indicating the immune-eliciting capacity of linear beta-1,3 glucans.

Linear β-1,3 Glucans Are Elicitors of Defense Responses in Tobacco

Discussion

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Implication

Key Points

Cite This Study

Linear β-1,3 Glucans Are Elicitors of Defense Responses in Tobacco

Discussion

Member takes

Implication

Key Points

Cite This Study

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