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The combination of liquid chromatography with electrochemical detection (LC-EC) is a powerful analytical tool for determining electroactive compounds in complex matrices. It has found numerous applications especially in the pharmaceutical and clinical areas. This experiment is intended to give students a practical experience with the LC-EC technique. The first part is designed to explore the electrochemistry of p-aminophenol (PAP), the analyte, while the second part deals with separation and identification of PAP in the presence of ascorbic acid and catechol. The improvement in detection limit with electrochemical detection compared to ultraviolet detection is also illustrated.
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Situmorang et al. (1998) studied this question.
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