Aflatoxin B1and total fumonisin contamination and their producing fungi in fresh and stored sorghum grain in East Hararghe, Ethiopia

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Natural contamination of sorghum grains by aflatoxin B1 and total fumonisin and their producing toxigenic fungi has been studied. A total of 90 sorghum grain samples were collected from small-scale farmers’ threshing floors and 5–6 months later from underground pits during 2013 harvest from three districts of East Hararghe, Ethiopia. Mycotoxin analysis was done using enzyme-linked immunosorbent assay (ELISA). The limits of detection were in the range 0.01–0.03 μg kg–1. The results revealed that all sorghum grain samples were contaminated with both Aspergillus and Fusarium species. Aflatoxin B1 was detected at levels ranging from