Development of Incompatibilities in Dogs by Repeated Infusions of Red Blood Cells.

In the course of an investigation on the influence of diet upon the regeneration of serum protein it was found, as has been reported by others,1 that with repeated plasmaphereses the hematocrit values tended to fall to anemia levels unless red blood cells from donors were injected periodically. Inasmuch as a fraction of the reinjected cells breaks down and liberates hemoglobin, which serves in the same capacity as dietary protein, an attempt was made to determine the degree of hemolysis in vivo in order to evaluate the liberated globin in terms of protein intake. Wu's2 ultra-micromethod for the determination of hemoglobin was adapted, as modified by Bing3 and McFarlane.4In all of the 4 experiments performed, the determination of hemoglobin on the citrated plasma, saline washing, and the modified Locke's solution5 in which the red cells were suspended for reinjection, indicated a remarkable uniformity in the hemolytic effects of these preliminary manipulations. The initial infusions of cells from the same ...