Lipoprotein Lipase Activity in Adipose Tissue from Hen, Chicken and Adult Human

Angervall, G. Lipoprotein lipase activity in adipose tissue from hen, chicken and adult human. Lipoprotein lipase was extracted from acetone powder of hen and chicken adipose tissue with dilute ammonia. The enzyme was tested in vitro in a system with milk cream suspension as a substrate and pure albumin or inhibitor‐freed plasma as a fatty acid acceptor. The activity was measured as a decrease in optical density in the system and as a release of unesterified fatty acids per 10 mg of enzyme extract. Lipoprotein lipase activity was three or four times greater in extracts of chicken adipose tissue as compared with those of hen. In adult human adipose tissue, however, there was no or insignificant lipoprotein lipase activity in three of four cases. — The role of inhibitors of lipoprotein lipase in connection with the method was discussed.