Key result
Detection of active IHHNV in shrimp was more accurate using single-step PCR amplification based on gene choice and reverse transcription coupled with PCR compared to universal primers.
Why the study?
Does single-step PCR based on gene choice or RT-PCR improve the accurate detection of active IHHNV compared to universal primers in Pacific whiteleg shrimp?
Does single-step PCR based on gene choice or RT-PCR improve the accurate detection of active IHHNV compared to universal primers in Pacific whiteleg shrimp?
Answers are grounded in this paper and cite their sources.
Alternative molecular strategies like gene-choice PCR and RT-PCR improve the diagnostic accuracy of active IHHNV in cultivated shrimp.
The Pacific whiteleg shrimp Litopenaeus vannamei (Penaeidae) is one of the most important cultivated species in world aquaculture. In Brazil, the northeastern states are home to the main shrimp producers. As shrimp aquaculture has expanded and intensified, diseases have progressively become one of the most serious threats to this industry. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is an enzootic viral agent in Brazilian shrimp farms. Its is usually diagnosed by histological methods. However, to detect sub-clinical or acute IHHNV infection, more refined methods based on molecular techniques have been utilized. We found that by using "universal" primers and a single-step PCR diagnostic test, it was difficult to distinguish between non-infective forms of the virus and active IHHNV. Detection of IHHNV was more accurate when we used two alternative molecular strategies, namely 1) single-step PCR amplification based on gene choice and 2) reverse transcription coupled with PCR.
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Teixeira et al. (2010) studied Infectious hypodermal and hematopoietic necrosis virus (IHHNV) infection. Single-step PCR amplification based on gene choice and reverse transcription coupled with PCR vs. "Universal" primers and single-step PCR was evaluated on Accurate detection of active IHHNV. Detection of active IHHNV in shrimp was more accurate using single-step PCR amplification based on gene choice and reverse transcription coupled with PCR compared to universal primers.
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