Answers are grounded in this paper and cite their sources.
The rate at which Mg2+ dissociates from the catalytically active magnesium-enzyme-substrate complex is much smaller than the catalytic rate in the phosphoglucomutase system. This observation was used in designing an all-or-none type of assay to distinguish between the free enzyme and its Mg2+ complex, and hence to measure the dissociation constant for the Mg2+ complex of phosphoglucomutase.
No takes yet. Share an insight, caveat, or question.
Ray et al. (1966) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: