What question did this study set out to answer?

To characterize exopolysaccharide (EPS) production across human Bifidobacterium breve isolates and determine how EPS modulates host innate and adaptive immune cell responses.

June 16, 2021Frontiers in MicrobiologyOpen Access

Bifidobacterium breve Exopolysaccharide Blocks Dendritic Cell Maturation and Activation of CD4+ T Cells

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To characterize exopolysaccharide (EPS) production across human Bifidobacterium breve isolates and determine how EPS modulates host innate and adaptive immune cell responses.
Screened human B. breve isolates for EPS production heterogeneity and evaluated immune responses in cultured human THP-1 monocytes.
Treated murine bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) with wild-type and isogenic EPS-deficient mutant strains (UCC2003 and JCM7017) to measure cytokine and surface marker gene expression.
Co-cultured BMDCs exposed to ovalbumin (OVA)-expressing wild-type or EPS-deficient B. breve strains with OT-II CD4+ T cells to evaluate antigen presentation.
Loss of EPS in B. breve UCC2003 increased BMDC expression of pro-inflammatory cytokine genes (Tnfa, Il6, Il12a, and Il23a) compared to both untreated and wild-type controls.
EPS-deficient strains of both UCC2003 and JCM7017 elevated BMDC maturation marker expression (Cd80, Cd83, and Cd86) relative to untreated BMDCs.
OVA-expressing EPS-deficient B. breve strains stimulated OVA-specific OT-II CD4+ T-cell activation during BMDC co-culture, whereas wild-type EPS-producing strains completely blocked activation.

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Implication

In vitro study reveals that Bifidobacterium breve exopolysaccharide suppresses dendritic cell maturation and T-cell activation in cell cultures, indicating a mechanism for gut immune tolerance.

Cite This Study

Hickey et al. (2021) studied this question.

synapsesocial.com/papers/6a9e350ee2d0fc9581be618d https://doi.org/10.3389/fmicb.2021.653587

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Implication

In vitro study reveals that Bifidobacterium breve exopolysaccharide suppresses dendritic cell maturation and T-cell activation in cell cultures, indicating a mechanism for gut immune tolerance.

Bifidobacterium breve Exopolysaccharide Blocks Dendritic Cell Maturation and Activation of CD4+ T Cells

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Discussion

Implication

Cite This Study

Bifidobacterium breve Exopolysaccharide Blocks Dendritic Cell Maturation and Activation of CD4+ T Cells

Key Points

Discussion

Implication

Cite This Study

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