Electrochemical Immunosensor Based on Metal Nanoparticles for Cardiac Myoglobin Detection in Human Blood Plasma

Cardiac myoglobin detection was based on direct electron transfer between the Fe(III)‐heme and the electrode surface that was modified with metal nanoparticles stabilized by didodecyldimethylammonium bromide and antibodies. Gold, silver and copper nanoparticles were tested as catalysts of the Fe(III)/Fe(II) electrode process. All experiments were carried out with human blood plasma samples of healthy donors and patients with acute myocardial infarction. The method proposed does not require labeled secondary antibodies. Immunosensor has a detection limit of 5 ng/mL and a broad range of working concentrations. The whole procedure takes 20 minutes and can be used to establish the diagnosis of acute myocardial infarction.