Key result
Cofilin 1 expression was higher in bladder cancer tissues than paracancerous tissues, and its downregulation in RT4 cells inhibited proliferation and migration while increasing apoptosis.
Cofilin 1 is overexpressed in human bladder cancer and its downregulation inhibits cancer cell progression in vitro, suggesting it as a potential therapeutic target.
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Cofilin 1 expression may mark bladder cancer progression; leaves open any clinical or therapeutic role pending validation.
PURPOSE: Cofilin 1 is a type of cytoskeletal protein. The overexpression of this gene has been regarded to hold a special relationship with the development and progress of some cancers. However, the detailed position of Cofilin 1 in human bladder cancer has not been investigated intensively. METHODS: In this study, we mainly explored the relationship between human bladder cancer and the expression of Cofilin 1. The expression of Cofilin 1 in bladder cancer tissues and paracancerous tissues of patients was evaluated with quantitative polymerase chain reaction, Western blot, and immunohistochemical staining. Downregulation of Cofilin 1 expression model was established with siRNA in human RT4 bladder cancer cell line, and the changing cell viability was analyzed to determine the role of Cofilin 1 in human bladder cancer. RESULTS: Our results showed that the expression of Cofilin 1 was much higher in both RNA level and protein level in human bladder cancer tissues than paracancerous tissues for 3 patients. Downregulation of Cofilin 1 expression could inhibit cell proliferation, cell migration, cell adhesion, and colony formation ability, and increase the percentage of cell apoptosis in RT4 cells. CONCLUSIONS: Our study indicates that Cofilin 1 holds an important position in the development and progression of human bladder cancer, and this gene might become a novel target in the diagnosis and treatment of human bladder cancer.
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Wang et al. (2016) studied Human bladder cancer (n=3). Cofilin 1 expression vs. Paracancerous tissues and control cells was evaluated on Cofilin 1 expression levels and cell viability, proliferation, migration, and apoptosis. Cofilin 1 expression was higher in bladder cancer tissues than paracancerous tissues, and its downregulation in RT4 cells inhibited proliferation and migration while increasing apoptosis.
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