Key result
Brownian dynamics simulations demonstrated that crosslinking of a meshwork of nonpolymerizing actin filaments can produce compressive forces up to several thousand pico-Newtons.
Crosslinking of actin networks can produce significant compressive forces, providing a mechanism for membrane deformation during clathrin-mediated endocytosis in yeast.
Actin crosslinking may generate forces for yeast endocytosis; simulation results leave open in vivo relevance.
Actin has been shown to be essential for clathrin-mediated endocytosis in yeast. However, actin polymerization alone is likely insufficient to produce enough force to deform the membrane against the huge turgor pressure of yeast cells. In this paper, we used Brownian dynamics simulations to demonstrate that crosslinking of a meshwork of nonpolymerizing actin filaments is able to produce compressive forces. We show that the force can be up to several thousand pico-Newtons if the crosslinker has a high stiffness. The force decays over time as a result of crosslinker turnover, and is a result of converting chemical binding energy into elastic energy.
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Ma et al. (2019) studied this question. Crosslinking of a meshwork of nonpolymerizing actin filaments was evaluated on Compressive force production. Brownian dynamics simulations demonstrated that crosslinking of a meshwork of nonpolymerizing actin filaments can produce compressive forces up to several thousand pico-Newtons.
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