Changes in the division rate of bone marrow cells in Wistar rats after exposure to nanosecond microwave pulses of different intensities

One of the promising areas of regenerative technologies is the stimulation of proliferative activity of stem cells and, accordingly, the rate of their self-renewal, by exposure to nanosecond repetitive pulsed microwave radiation (RPMs). The article presents data on the effect of nanosecond RPMs on the rate of division of mesenchymal stem cells from the femur of Wistar rats. Material and methods. Data are presented on the effect of nanosecond repetitively pulsed microwave radiation carrier frequency of 9.4 GHz, pulse repetition rate of 13 Hz, 50 pulses, peak power flux density of 140, 210, and 310 W/cm2, absorbed energy value in 50 pulses at a depth of 1 cm, respectively 699, 1049 and 1549×10–6 J/cm3 on the division rate of mesenchymal stem cells from the femur of laboratory rats Wistar. The effect of the exposure was assessed by the change in the number of cells in the culture 24 and 72 hours after a single irradiation with RPMs with different intensity. Results. Depending on the intensity of RPM, both an increase and inhibition of cell division rate were observed, that is, the response had a phasic character. The most pronounced stimulating acceleration of cell division is exerted by RPMs with a peak power flux density of 140 W/cm2, and the effect is realized at the maximum rate after 24 h. Conclusions. The most probable biophysical and physiological mechanisms for the formation of the effects of changes in the rate of self-renewal of stem cells induced by RPMs are considered.