A reliable HPLC analysis of glucose-fructose-mannose mixtures, occurring in the commerical alkali catalysed production of High Fructose Syrup (HFS) from glucose is developed. As stationary phase a mechanically stable, cheap and easily available unmodified silica is used. The mobile phase is acetonitrile containing 0.1% w of water. The separation is complete within 25 minutes, at a pressure drop of 100 bar over the column.
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Olst et al. (1979) studied this question.
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