What question did this study set out to answer?

To determine whether the stimulatory effects of epinephrine, glucagon, and adrenocorticotropin on glucose uptake and oxidation depend on their lipolytic actions in isolated rat adipocytes.

July 1, 1969Journal of Biological ChemistryOpen Access

Independence of the Effects of Epinephrine, Glucagon, and Adrenocorticotropin on Glucose Utilization from Those on Lipolysis in Isolated Rat Adipose Cells

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To determine whether the stimulatory effects of epinephrine, glucagon, and adrenocorticotropin on glucose uptake and oxidation depend on their lipolytic actions in isolated rat adipocytes.
Isolated epididymal adipocytes from rats and stimulated them with epinephrine, glucagon, or adrenocorticotropin (ACTH) in the presence or absence of bovine serum albumin.
Evaluated lipolysis alongside glucose uptake and oxidation after treatment with inhibitors and modulators, including prostaglandin E1, nicotinic acid, caffeine, theophylline, and beta-adrenergic blockers (propranolol and Ko 592).
Prostaglandin E1 and nicotinic acid blocked epinephrine-stimulated lipolysis without altering its stimulation of glucose metabolism, and fatty acid accumulation was not required for glucose entry or oxidation.
Beta-adrenergic blockers inhibited both lipolysis and glucose oxidation induced by epinephrine, but only suppressed the lipolytic effects—not the glucose oxidation effects—of glucagon and ACTH.
Prostaglandin E1 only partially suppressed hormone-stimulated lipolysis even at excessive doses, whereas beta-adrenergic blockers produced complete inhibition, indicating multiple activation sites within the adenyl cyclase system.

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Implication

Experimental study demonstrates independent regulation of glucose utilization and lipolysis in rat adipocytes, indicating distinct receptor-mediated pathways.

Cite This Study

Blecher et al. (1969) studied this question.

synapsesocial.com/papers/6a9e4ae20802d747abf6d9ae https://doi.org/10.1016/s0021-9258(18)83389-0

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Implication

Experimental study demonstrates independent regulation of glucose utilization and lipolysis in rat adipocytes, indicating distinct receptor-mediated pathways.

Independence of the Effects of Epinephrine, Glucagon, and Adrenocorticotropin on Glucose Utilization from Those on Lipolysis in Isolated Rat Adipose Cells

Key Points

Discussion

Implication

Cite This Study

Independence of the Effects of Epinephrine, Glucagon, and Adrenocorticotropin on Glucose Utilization from Those on Lipolysis in Isolated Rat Adipose Cells

Key Points

Discussion

Implication

Cite This Study

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