Significant gains in LC–NMR signal-to-noise ratio are obtained by selective concentration of chromatographic peaks on a chromatographic column using D2O to fix the sample on the solid phase. A deuterated organic solvent is used to remove the sample from the column and into the NMR flow cell, which removes the need for presaturation of solvent resonances. Deuterated solvents unrelated to the original eluent are used in order to minimize spectral overlap between solvent and solute.
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Griffiths et al. (1998) studied this question.