Key result
The methyl ester of amphotericin B (100 microgram/ml) increased the infectivity of encephalomyocarditis virus RNA for L929 cells by 10- to 100-fold.
Amphotericin B Methyl Ester significantly enhances the infectivity of encephalomyocarditis virus RNA in L929 cells, likely by increasing intracellular penetration.
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Amphotericin B methyl ester may aid viral RNA delivery in vitro; leaves open relevance to in vivo models or applications.
The methyl ester of amphotericin B (AmBME), a macrolide polyene antibiotic, enhanced the infectivity of encephalomyocarditis (EMC) virus RNA for L929 cells. AmBME alone (100 microgram/ml) resulted in increases in EMC virus RNA infectivity of 10- to 100-fold. Addition of DEAE dextran at concentrations (5 microgram/ml), which alone slightly suppressed EMC virus RNA infectivity, further augmented the effects of AmBME (augmentation in infectivity up to 750-fold). AmBME did not inhibit RNase, did not enhance EMC virus infectivity and increased infectivity of EMC virus RNA which was already cell-associated. The polyenes are probably acting by increasing intracellular penetration of polyribonucleotides.
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Borden et al. (1979) studied Encephalomyocarditis (EMC) virus RNA infectivity. Methyl ester of amphotericin B (AmBME) was evaluated on Infectivity of EMC virus RNA for L929 cells (10- to 100-fold increase). The methyl ester of amphotericin B (100 microgram/ml) increased the infectivity of encephalomyocarditis virus RNA for L929 cells by 10- to 100-fold.
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