Key result
Pretreatment of L cells with insulin and infection in the presence of Amphotericin B methyl ester markedly and dose-dependently increased viral RNA-induced interferon production.
Pretreatment of L cells with insulin and infection in the presence of Amphotericin B methyl ester enhances viral RNA-induced interferon production.
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May potentiate interferon responses in vitro; leaves open translation to antiviral strategies in vivo.
A study was initiated to develop a more efficient method of producing high-titered L cell interferon. The induction of interferon by viral infectious RNA could be augmented by pretreating the cell cultures with insulin and infecting in the presence of Amphotericin B methyl ester (AmBME). L cells were treated for 24 hr with different concentrations of insulin and then challenged with MM virus RNA suspended in a balanced salt solution with and without AmBME. The fluids were collected 48 hr after infection and assayed for interferon activity. Interferon production was markedly increased in insulin-treated cultures and that the increase was dose-dependent.
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Giron et al. (1981) studied this question. Insulin and Amphotericin B methyl ester (AmBME) vs. without AmBME / untreated was evaluated on Interferon production. Pretreatment of L cells with insulin and infection in the presence of Amphotericin B methyl ester markedly and dose-dependently increased viral RNA-induced interferon production.
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