What question did this study set out to answer?

To establish an efficient method for collecting and dual-staining thin epoxy resin sections for light microscopic evaluation.

January 1, 1975Journal of Microscopy

Handling and staining epoxy resin sections for light microscopy

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Authors

IRI. M. RobertsRutgers, The State University of New Jersey AHA. M. HutchesonUniversity of Dundee

Discussion

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Implication

Methodological study demonstrates reliable collection and dual staining of epoxy resin sections, indicating an improved workflow for light microscopy preparation.

Key Points

To establish an efficient method for collecting and dual-staining thin epoxy resin sections for light microscopic evaluation.
Collected 0.1–1.0 µm thick epoxy resin sections from an ultramicrotome using coverslip strips and dried them on a hot plate.
Treated dried sections with periodic acid, followed by sequential bulk staining with basic fuchsin at 70°C and alkaline methylene blue at room temperature in custom troughs.
Facilitated accurate collection of serial sections without disrupting the ultramicrotome sectioning process.
Provided effective differential staining across multiple specimen types and epoxy resin formulations that remained stable for more than one year.

Cite This Study

Roberts et al. (1975) studied this question.

synapsesocial.com/papers/6a9e513e424eb4712abe5b7d https://doi.org/10.1111/j.1365-2818.1975.tb04543.x

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Implication

Methodological study demonstrates reliable collection and dual staining of epoxy resin sections, indicating an improved workflow for light microscopy preparation.

Handling and staining epoxy resin sections for light microscopy

Discussion

Member takes

Implication

Key Points

Cite This Study

Handling and staining epoxy resin sections for light microscopy

Discussion

Member takes

Implication

Key Points

Cite This Study

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