Key result
A novel method was described to control intracellular ionic conditions, enabling the examination of the forward (Ca2+ extrusion) mode of exchange of NCKX2.
This study provides a novel methodological approach to measure the forward Ca2+ extrusion mode of NCKX2, overcoming previous limitations of relying on reverse mode assays.
Enables targeted study of NCKX2 forward-mode Ca2+ extrusion in vitro; leaves open relevance to cardiac ion handling in vivo.
Na+/Ca2+-K+ exchangers (NCKX) are plasma membrane transporters that are thought to mainly mediate Ca2+ extrusion (along with K+) at the expense of the Na+ electrochemical gradient. However, because they are bidirectional, most assays have relied on measuring their activity in the reverse (Ca2+ import) mode. Herein we describe a method to control intracellular ionic conditions, and examine the forward (Ca2+ extrusion) mode of exchange of NCKX2.
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Altimimi et al. (2007) studied this question. Method to control intracellular ionic conditions was evaluated on Forward (Ca2+ extrusion) mode of exchange of NCKX2. A novel method was described to control intracellular ionic conditions, enabling the examination of the forward (Ca2+ extrusion) mode of exchange of NCKX2.
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