Key result
Exosomes released from hypoxic and irradiated A549 cells significantly promoted tumor cell proliferation, migration, invasion, and HUVEC angiogenesis, driven by the ANGPTL4 protein.
Why the study?
The function of exosomal proteins in cancer progression under different oxygen conditions following irradiation was poorly understood.
Do exosomes derived from hypoxic and irradiated lung cancer cells promote tumor progression and angiogenesis?
Do exosomes derived from hypoxic and irradiated lung cancer cells promote tumor progression and angiogenesis?
Exosomes derived from hypoxic and irradiated lung cancer cells promote tumor progression and angiogenesis, highlighting ANGPTL4 as a potential diagnostic biomarker and therapeutic target.
May implicate ANGPTL4 in radiation resistance of lung cancer cells; hypothesis-generating and leaves open clinical relevance.
Numerous studies have shown that exosomes play important roles in tumor biology development. However, the function of exosomal protein in cancer progression under different oxygen condition after irradiation is poorly understood. In this study, non-small cell lung cancer (NSCLC) A549 cells were γ-ray irradiated under normoxic or hypoxic conditions, then the exosomes released from the irradiated cells were collected and co-cultured with nonirradiated A549 cells or human umbilical vein endothelial cells (HUVECs). It was found that the exosomes significantly promoted the proliferation, migration and invasion of A549 cells as well as the proliferation and angiogenesis of HUVECs. Moreover, the exosomes released from hypoxic cells and/or irradiated cells had more powerful driving force in tumor progression compared to that generated from normoxia cells. Meanwhile, the proteins contained in the exosomes derived from A549 cells under different conditions were detected using tandem mass tag (TMT), and their expression profiles were analyzed. It was found that the exosome-derived protein of angiopoietin-like 4 (ANGPTL4) contributed to the migration of A549 cells as well as the angiogenesis of HUVECs, suggesting its potential as an effective diagnostic biomarker of metastasis and even a therapeutic target of lung cancer.
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Mo et al. (2020) studied Non-small cell lung cancer (NSCLC). Exosomes from γ-ray irradiated A549 cells under hypoxic conditions vs. Exosomes from normoxia cells or nonirradiated cells was evaluated on Proliferation, migration, and invasion of A549 cells and angiogenesis of HUVECs. Exosomes released from hypoxic and irradiated A549 cells significantly promoted tumor cell proliferation, migration, invasion, and HUVEC angiogenesis, driven by the ANGPTL4 protein.
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