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An increase in Ca2+ from a pCa of 9 to 4 induced large-scale mechanical changes in cross-linked troponin C films, causing several percent shrinkage of the unloaded films.
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Ca2+ binding to troponin C induces large-scale mechanical changes and shrinkage in cross-linked TnC films, demonstrating its structural regulatory function.
Changes in troponin C (TnC) conformation upon Ca(2+) binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca(2+)-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-linked with glutaraldehyde, and isometric tension and stiffness measured as a function of pCa. An increase in Ca(2+) from a pCa of 9 to 4 induced large-scale mechanical changes in the TnC films causing several percent shrinkage of the unloaded films. This shrinkage could be partially assigned to Ca(2+) binding to the Ca(2+)/Mg(2+) sites of TnC.
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Bukatina et al. (2002) studied this question. Ca2+ increase was evaluated on Mechanical changes (isometric tension, stiffness, and shrinkage). An increase in Ca2+ from a pCa of 9 to 4 induced large-scale mechanical changes in cross-linked troponin C films, causing several percent shrinkage of the unloaded films.
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