Cellular localization and photodynamic effect of chlorin e 6-monoclonal antibody conjugate

Chlorin e6(Ce6) was conjugated with anti-tumor monoclonal antibody ( IgG ) to increase its binding affinity for tumors. Ce 6 was activated by N -hydroxysulfosuccinimide and conjugated with IgG via peptide bonds, and Ce 6 molecules were conjugated with IgG ( IgG - Ce 6) and their binding affinities to tumor cells were investigated. Intracellular localization of IgG - Ce 6 was observed, and IgG - Ce 6 was accumulated in tumor cells much higher than Ce 6, indicating that the IgG - Ce 6 has specific binding affinity to tumor cells. The effective photocytotoxicity of the cells with IgG - Ce 6 is caused by the high accumulation of IgG - Ce 6 in tumor cells.