Key result
Sepsis significantly prolonged atrial action potential duration (106.40 vs 86.60 ms) and increased heart rate (361.10 vs 348.60 bpm) compared to control in rats.
Why the study?
The effect of sepsis on rat serotonin responses and cardiac action potentials remained to be investigated.
Does sepsis alter cardiac action potentials and serotonin responses in a rat model?
Does sepsis alter cardiac action potentials and serotonin responses in a rat model?
Absolute Event Rate: 106.4% vs 86.6%
p-value: p=<0.01
Sepsis induces prolonged atrial action potentials, increased heart rate, and elevated myocardial serotonin expression in rats, suggesting serotonin may contribute to sepsis-induced cardiac dysfunction.
Sepsis may alter atrial electrophysiology in rats; leaves open human arrhythmia relevance and requires clinical validation.
The current study aimed to investigate the effect of sepsis on rat serotonin (5‑HT) responses and cardiac action potentials. A total of 20 rats were randomly divided into a sepsis and control group (each, n=10). Rat hearts were harvested and perfused using the Langendorff method 18‑h after the induction of sepsis, which was assessed using cecal puncture. Cardiac action potential was subsequently measured using a multichannel electrophysiology instrument. Immunohistochemistry and quantitative analysis were performed to identify the effect of sepsis on myocardial 5‑HT expression. The results revealed that mitochondrial changes were present in septic rat hearts. Heart rate (361.10±12.29 bpm vs. 348.60±12.38 bpm; P<0.05) was significantly higher, atrial action potential duration (106.40±2.95 ms vs. 86.60±4.12 ms; P<0.01) was significantly longer and the area (0.62±0.06 µm2 vs. 0.39±0.05 µm2; P<0.05) and number (0.92±0.02/field vs. 0.46±0.01/field; P<0.01) of myocardial cells were significantly higher in the septic compared with the control group. These results demonstrated that 5‑HT prolongs the atrial action potential, increases heart rate and aggravates myocardial injury, indicating that it may therefore be one of the factors that leads to cardiac dysfunction in sepsis.
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Liu et al. (2019) studied Sepsis (n=20). Sepsis induction (cecal ligation and puncture) vs. Sham procedure was evaluated on Atrial action potential duration (p=<0.01). Sepsis significantly prolonged atrial action potential duration (106.40 vs 86.60 ms) and increased heart rate (361.10 vs 348.60 bpm) compared to control in rats.
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