Key result
Recombinant DHV-1 VP1 protein, particularly when combined with the adjuvant bursin, induced significant IgG antibody production, lymphocyte proliferation, and virus-neutralizing antibodies in ducklings.
Recombinant VP1 protein of DHV-1 expressed in P. pastoris induces a significant immune response in ducklings, especially with an adjuvant, suggesting potential as a subunit vaccine.
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Supports preclinical VP1 subunit vaccine development for DHV-1; leaves open protective efficacy and field translation in ducks.
The VP1 gene of duck hepatitis virus type 1 (DHV-1) strain VJ09 was amplified by reverse transcription PCR from the liver of a duckling with clinical symptoms of viral hepatitis. The resulting VP1 cDNA was 720 bp in length and encoded a 240-amino-acid protein. In VP1 gene-based phylogenetic analysis, the VJ09 strain grouped with DHV-1 genotype C. The VP1 gene was inserted into the expression vector pPICZαA and expressed in Pichia pastoris. The expressed VP1 protein was purified and identified by western blot analysis. To evaluate the recombinant VP1's immunogenic potential in ducklings, the antibodies raised in the immunized ducklings were titrated by ELISA, and lymphocyte proliferation and virus neutralization assays were performed. The results show that the recombinant VP1 protein induced a significant immune response in ducklings and this could be a candidate for the development of a subunit vaccine against DHV-1 genotype C.
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Wang et al. (2014) studied Healthy ducklings (DHV-1 vaccination) (n=120). Recombinant DHV-1 VP1 protein vs. PBS was evaluated on Immunogenicity (IgG antibodies, lymphocyte proliferation, and virus neutralization) (p=≤0.05). Recombinant DHV-1 VP1 protein, particularly when combined with the adjuvant bursin, induced significant IgG antibody production, lymphocyte proliferation, and virus-neutralizing antibodies in ducklings.
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