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Cytochemical reaction for Δ5-3/3- hydroxysteroid dehydrogenase was studied in monolayers of testicular cells grown in vitro. Cells in cultures established from testicular tissue of immature rats were essentially negative for Δ6-3/3β-hydroxysteroid dehydrogenase. Cells in cultures established from testicular tissue of similar animals pretreated with human chorionic gonadotropin (HCG) were strongly positive for Δ5-3/3β-hydroxysteroid dehydrogenase. Addition of HCG to the culture medium failed to induce a positive reaction for this enzyme. These studies show that testicular cells which may be active in steroid biosynthesis can be grown in vitro. (Endocrinology79: 406,1966)
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Steinberger et al. (1966) studied this question.
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