Mesohalobacter salilacus sp. nov., a novel halotolerant, flexirubin-type pigment-producing bacterium of the family Flavobacteriaceae

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A Gram-staining-negative, non-motile, aerobic, rod-shaped bacterium, designated 14752T, was isolated from a saline lake in Xinjiang Uygur Autonomous Region, China. The strain was subjected to a taxonomic study using a polyphasic approach. Strain 14752T was able to grow at 4–40 ℃ (optimum 28 ℃), pH 6.0–7.5 (optimum 7.0) and with 1.0–9.0% NaCl (optimum 3–4%, w/v). Phylogenetic and sequence similarity analysis of 16S rRNA gene sequences indicated that strain 14752T was grouped with Mesohalobacter halotolerans WDS2C27T, with the highest similarity of 98.13%. In comparison, this similarity between strain 14752T and other valid published species was less than 92%. The average nucleotide identity based on blast alignments, digital DNA–DNA hybridization and average amino identity values between strain 14752T and M. halotolerans WDS2C27T were 80.13%, 22.60% and 83.91%, respectively. Chemotaxonomic analysis showed that the sole respiratory quinone was menaquinone 6 (MK-6), the major fatty acids included anteiso-C15:0, iso-C15:0 and iso-C15:1 G. The major polar lipids included phosphatidylethanolamine, two uncharacterized aminolipids, one uncharacterized amino phospholipid, one uncharacterized phospholipid and one uncharacterized lipid. The genomic DNA G+C content of the strain 14752T was 34.1 mol%. According to the physiological, genotypic, genomic and chemotaxonomic features revealed in this investigation, it is recommended that strain 14752T represents a novel species of the genus Mesohalobacter, for which the name Mesohalobacter salilacus sp. nov. is proposed. The type strain is 14752T (=KCTC 72044T=MCCC 1K03765T).