Optimization of an anti Staphylococcus antibiotic produced by tropical soil dwelling Streptomyces parvulus

An antibiotic produced by strain Streptomyces parvulus showing activity against Staphylococcus citreus was subjected to various optimization parameters for enhancing its production. Nutritional and physiological parameters produced by S. parvulus under shaken flask conditions were determined. Optimization of these parameters led to 11% increase in antibiotic activity with a mean zone of inhibition of 42 mm. Highest antibiotic production was obtained at 250 rpm for 14 days with optimum temperature of 28°C and pH 7. Kuster#x2019;s modified medium containing glycerol 0.7% (v/v), casein 0.03% (w/v), NaCl 0% (w/v), phosphate 0.25% (w/v), KNO3 0.1% (w/v) and CaCO3 0.0015% (w/v) concentration was found ideal.