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August 18, 2005Journal of BacteriologyOpen Access

Identification of theMycobacterium tuberculosisSUF Machinery as the Exclusive Mycobacterial System of Fe-S Cluster Assembly: Evidence for Its Implication in the Pathogen's Survival

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Authors

GHGaëlle HuetLaboratoire des Interactions Plantes Micro-OrganismesMDMamadou DafféCentre National de la Recherche ScientifiqueISIsabelle SavesUniversity of Antananarivo

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Implication

Molecular investigation reveals the SUF machinery as the sole iron-sulfur cluster assembly pathway in Mycobacterium tuberculosis, highlighting Pps1 as a promising drug target.

Key Points

  • To characterize the Mycobacterium tuberculosis pps1 locus and evaluate the role of the SUF machinery in mycobacterial iron-sulfur cluster assembly and pathogen survival.
  • Performed in silico sequence and orthology analyses of the conserved seven-gene pps1 locus containing an intein coding sequence.
  • Characterized protein-protein interactions among encoded Suf-like components to assess complex assembly.
  • Evaluated the essentiality of the suf operon and the absence of alternative iron-sulfur assembly systems using Mycobacterium smegmatis.
  • Identified mature Pps1 as an ortholog of SufB encoded within a conserved seven-gene operon that functions as the SUF machinery in Mycobacterium tuberculosis.
  • Confirmed direct physical interactions among encoded Suf-like proteins, validating the operon's biochemical functionality.
  • Demonstrated the essentiality of the suf operon in mycobacteria, establishing the pathway as the pathogen's sole system for iron-sulfur cluster biogenesis and survival under oxidative stress and iron limitation.

Cite This Study

Huet et al. (2005) studied this question.

synapsesocial.com/papers/6a9e84b3c648cf9f6a40d04ehttps://doi.org/10.1128/jb.187.17.6137-6146.2005
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