The Activity of the Pyrimidine Biosynthetic Pathway in MGH-U1 Transitional Carcinoma Cells Grown in Tissue Culture

No AccessJournal of Urology1 Jun 1977The Activity of the Pyrimidine Biosynthetic Pathway in MGH-U1 Transitional Carcinoma Cells Grown in Tissue Culture David R. Evans, Robert J. Irwin, Pamela A. Havre, John G. Bouchard, Tetsuro Kato, and George R. Prout David R. EvansDavid R. Evans Current address: Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201. More articles by this author , Robert J. IrwinRobert J. Irwin Current address: Division of Urology, Department of Surgery, University of Mississippi Medical Center, Jackson, Mississippi 39216. More articles by this author , Pamela A. HavrePamela A. Havre Current address: Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201. More articles by this author , John G. BouchardJohn G. Bouchard Current address: Dartmouth College, Hanover, New Hampshire 03755. More articles by this author , Tetsuro KatoTetsuro Kato Current address: Department of Urology, Akita University School of Medicine, Akita (34)111, Japan 010. More articles by this author , and George R. ProutGeorge R. Prout More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)58598-5AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail MGH-U1, a new cell line of transitional carcinoma grown in tissue culture, is described. The growth characteristics, growth kinetics and the activity of the pyrimidine biosynthetic pathway are outlined. The pathway activity was measured in intact MGH-U1 cells and 4 of the biosynthetic enzymes were assayed in cellular extracts. The level of activity of the pathway remains unaltered in cultures that range in density from 104to 4 × 105 cells per cm.2 and the cells actively incorporate precursors into macromolecules throughout the entire range of densities investigated. These results differ from those reported previously, in which the synthesis of ribonucleic acid, deoxyribonucleic acid and protein is reduced more than 90 per cent in stationary suspension cultures of Novikoff rat hepatoma cells and in confluent monolayers of human fibroblasts that exhibit contact inhibition. The levels of 3 of the 4 enzymes assayed in MGH-U1 cells are reduced 50 to 60 per cent when the cells undergo the transition from rapid exponential growth to confluence but are still present in levels that are adequate to sustain a high level of pyrimidine biosynthesis. The compound, N-(phosphonacetyl)-L-aspartate was found to inhibit completely aspartate transcarbamylase, the second enzyme of the pathway, in cellular extracts of MGH-U1 cells and greatly retarded the growth of these cells when added to the culture medium. The latter effect could be circumvented by the simultaneous administration of uridine, which is used via the salvage pathways for the continuing requirement of the cells for pyrimidine nucleotides. © 1977 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByHawkyard S, James K, Prescott S, Jackson A, Ritchie A, Smyth J and Chisholm G (2018) The Effects of Recombinant Human Intereron-Gamma on a Panel of Human Bladder Cancer Cell LinesJournal of Urology, VOL. 145, NO. 5, (1078-1081), Online publication date: 1-May-1991. Volume 117Issue 6June 1977Page: 712-719 Advertisement Copyright & Permissions© 1977 by The American Urological Association Education and Research, Inc.MetricsAuthor Information David R. Evans Current address: Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201. More articles by this author Robert J. Irwin Current address: Division of Urology, Department of Surgery, University of Mississippi Medical Center, Jackson, Mississippi 39216. More articles by this author Pamela A. Havre Current address: Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201. More articles by this author John G. Bouchard Current address: Dartmouth College, Hanover, New Hampshire 03755. More articles by this author Tetsuro Kato Current address: Department of Urology, Akita University School of Medicine, Akita (34)111, Japan 010. More articles by this author George R. Prout More articles by this author Expand All Advertisement PDF DownloadLoading ...