The Effects of Changes in Hydrogen Ion Concentration on the Blood Flow of Morphinized Dogs

HYDROGEN ION CONCENTRATION AND BLOOD FLOWOne of the arterial samples was used for pH determination accord- ing to Hawkins' (1923) modification of Cullen's (1922) colorimetric method.In many of the experiments in which acid was injected slight laking of the blood occurred and accurate pH readings were difficult.Greater accuracy than --pH 0.03 is not assumed.However the changes found in H-ion concentration were much greater than the limits of error of the method.The oxygen and carbon dioxide content of the arterial and venous blood were determined.The circulatory minute volume was calcu- lated from the Fick formula: CC. 02 consumed per minute Number of cubic centimeters of blood Amount 02 taken up in lungs by 1 cc. of blood flowing through lungs per minuteAcid and alkali were injected into the femoral vein.In some experi- ments N/5 HCI was used, in others N/5, N/2.5 or N/1 lactic acid, N/l