Key result
The RNA-binding protein Arrest (Aret, Bruno) regulates alternative splicing of salm-dependent sarcomeric targets to enable myofibril maturation and sarcomere growth in Drosophila flight muscle.
The RNA-binding protein Arrest (Bruno) regulates alternative splicing essential for myofibril maturation in Drosophila, highlighting a conserved mechanism that may apply to mammalian muscle morphogenesis.
Answers are grounded in this paper and cite their sources.
No clinical implications yet; leaves open whether salm-Aret splicing control extends to vertebrate sarcomeres.
In Drosophila, fibrillar flight muscles (IFMs) enable flight, while tubular muscles mediate other body movements. Here, we use RNA-sequencing and isoform-specific reporters to show that spalt major (salm) determines fibrillar muscle physiology by regulating transcription and alternative splicing of a large set of sarcomeric proteins. We identify the RNA-binding protein Arrest (Aret, Bruno) as downstream of salm. Aret shuttles between the cytoplasm and nuclei and is essential for myofibril maturation and sarcomere growth of IFMs. Molecularly, Aret regulates IFM-specific splicing of various salm-dependent sarcomeric targets, including Stretchin and wupA (TnI), and thus maintains muscle fiber integrity. As Aret and its sarcomeric targets are evolutionarily conserved, similar principles may regulate mammalian muscle morphogenesis.
No takes yet. Share an insight, caveat, or question.
Spletter et al. (2014) studied Drosophila muscle development. RNA-binding protein Arrest (Aret, Bruno) was evaluated on Myofibril maturation and sarcomere growth of IFMs. The RNA-binding protein Arrest (Aret, Bruno) regulates alternative splicing of salm-dependent sarcomeric targets to enable myofibril maturation and sarcomere growth in Drosophila flight muscle.
Synapse has enriched 10 related papers. Consider them for comparative context: