Key result
Thrombopoietin greatly promotes the self-renewal and expansion of hematopoietic stem cells in vivo following marrow transplantation, with normal recipients showing 13- to 20-fold greater expansion than Tpo-null mice.
Why the study?
Does recombinant thrombopoietin improve hematopoietic stem cell self-renewal and expansion in lethally irradiated mice following marrow transplantation?
Population
C57BL/6 mice (Tpo-/- and Tpo+/+), lethally irradiated (1150 cGy) and transplanted with normal marrow cells.
Comparison
Recombinant murine thrombopoietin 80 ng… vs Tpo-/- mice given saline thrice weekly, or…
Design
Preclinical
Follow-up
up to 22 weeks
Authors
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Animal data on TPO-driven HSC expansion do not support clinical use; leaves open translation to human marrow transplantation.
Does recombinant thrombopoietin improve hematopoietic stem cell self-renewal and expansion in lethally irradiated mice following marrow transplantation?
Effect estimate: 13- to 20-fold greater expansion
Thrombopoietin greatly promotes the self-renewal and expansion of hematopoietic stem cells in vivo following marrow transplantation.
Fox et al. (2002) studied Hematopoietic stem cell transplantation. Thrombopoietin (TPO) vs. Tpo-null (Tpo-/-) mice without TPO was evaluated on Relative expansion of long-term repopulating hematopoietic stem cells (13- to 20-fold greater expansion). Thrombopoietin greatly promotes the self-renewal and expansion of hematopoietic stem cells in vivo following marrow transplantation, with normal recipients showing 13- to 20-fold greater expansion than Tpo-null mice.