HPLC Determination of Furfural and 5‐Hydroxymethylfurfural in Citrus Juices

A rapid sample clean‐up and chromatographic separation were devel‐ oped to determine both furfural and 5‐hydroxymetbylfurfural (HMF) in citrus juices. Furfural and HMF were isolated by using a C‐18 cartridge, and quantified by isocratic elution with acetonitrile/water (1Y85, v/v) as the mobile phase with a Radial‐PAK C‐18 column (100 × 8 mm i.d.). Detection was at 280 nm and the lower limit of detection for both compounds was 50 ppb. Recoveries between 94.0 and 101.2% were obtained for HMF added to orange juice. Recoveries of fmfural ranged between 96.8 and 103.9%.