The influence of ventilatory control on heart rate variability in children

The aim of this study was to determine the influence of breathing frequency and tidal volume on resting heart rate variability in children aged 9 years (n = 29) and 16 years (n = 19). Heart rate variability was measured in four conditions: (1) without the control of ventilation followed at random by (2) a fixed breathing frequency of 12 breaths x min(-1), (3) a breathing frequency of 12 breaths x min(-1) but with a fixed tidal volume of 30% vital capacity and (4) a fixed breathing frequency of 6 breaths x min(-1) and a tidal volume of 30% vital capacity. A total of 128 RR intervals (the time between two spikes in the heart rate) were detected and absolute high- and low-frequency spectral components were calculated using autoregressive modelling. The younger children were unable to control ventilation to achieve conditions 3 and 4; therefore, a 2 x 2 (group x condition) analysis of variance was used to analyse conditions 1 and 2. There were significant interactions between group and heart rate variability conditions for the low-frequency component and the ratio of low to high frequencies (P < 0.001). The main effect for condition showed that at 12 breaths x min(-1) with no fixed tidal volume there was a significantly higher standard deviation of the RR interval, total power and high-frequency (P< 0.01) and low-frequency spectral components (P < 0.05) than in the condition with no ventilatory control. Across the four breathing conditions for the older participants, the high-frequency spectral component was significantly higher in the condition at 6 breaths x min(-1) with a fixed tidal volume than in that with no ventilatory control (P < 0.005); the ratio of high to low frequencies was significantly lower for the spontaneous condition than those performed at 12 breaths x min(-1) (P < 0.001). The results provide evidence of the need for ventilatory control when assessing short-term resting heart rate variability in children.