Regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking through PKA phosphorylation of the Glu receptor 1 subunit
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Discussion
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Implication
Laboratory study reveals PKA phosphorylation of GluR1 serine 845 controls AMPA receptor membrane insertion and endocytosis in neurons, highlighting a mechanism for synaptic plasticity.
Key Points
- To determine whether direct phosphorylation of the AMPA receptor GluR1 subunit by PKA regulates cell-surface receptor trafficking and distribution.
- Assessed AMPA receptor membrane trafficking and cell-surface expression following PKA-mediated phosphorylation.
- Analyzed the effects of GluR1 serine 845 phosphorylation and dephosphorylation on receptor insertion, endocytosis, and NMDA-induced internalization.
- Phosphorylation of GluR1 at serine 845 by PKA is necessary to elevate AMPA receptor surface expression by promoting insertion and reducing endocytosis.
- Dephosphorylation of GluR1 at serine 845 directly initiates NMDA-dependent AMPA receptor internalization, driving synaptic plasticity.
Cite This Study
Man et al. (2007) studied this question.