Key result
High-performance liquid chromatography with fluorescence detection can reliably quantitate zolpidem in human plasma with a lower limit of 1-2.5 ng/mL for single-dose pharmacokinetic studies.
The described HPLC method with fluorescence detection is applicable for quantifying zolpidem in human plasma for single-dose pharmacokinetic studies.
Facilitates single-dose zolpidem pharmacokinetic studies in research; leaves open validation for clinical therapeutic monitoring.
Zolpidem, an imidazopyradine hypnotic agent, can be quantitated by high-performance liquid chromatography (HPLC) with fluorescence detection. After the addition of a structurally related internal standard (propyl-zolpidem), plasma samples were double-extracted at neutral pH with toluene-isoamyl alcohol or benzene-isoamyl alcohol. The organic extracts were evaporated to dryness, reconstituted with mobile phase, and analyzed by HPLC using a C-18 reversed-phase column, a mobile phase of acetonitrile-50mM potassium dihydrogen phosphate (50:50), and fluorescence detection with excitation and emission wavelengths of 254 and 390 nm, respectively. The lower limit of reliable quantitation was in the range of 1-2.5 ng/mL. The method is applicable to single-dose pharmacokinetic studies of zolpidem in humans.
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Durol et al. (1997) studied this question. High-performance liquid chromatography with fluorescence detection was evaluated on Lower limit of reliable quantitation. High-performance liquid chromatography with fluorescence detection can reliably quantitate zolpidem in human plasma with a lower limit of 1-2.5 ng/mL for single-dose pharmacokinetic studies.
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