Enantiomeric separation by capillary electrophoresis of di- and tri-peptides derivatized with 9-fluorenylmethyl chloroformate using vancomycin as chiral selector

Enantiomeric separations of chiral di- and tri-peptides derivatized with 9-fluorenylmethyl chloroformate were carried out by capillary electrophoresis using vancomycin as a chiral selector. The mobilities of vancomycin were determined in phosphate and HEPES buffer by means of a factorial design. The zero mobility of vancomycin was found to be, to a small extent, dependent on buffer type, buffer concentration, and the presence of organic modifier. Application of a pH above zero mobility of vancomycin resulted in highly efficient separation of 15 di- and tri-peptides in less than 12 min. The influence of 2-propanol, buffer types, and sodium dodecyl sulfate on selectivity and efficiency was also investigated.