Key result
RT-qPCR and ERNS antigen ELISAs provided reliable BVDV detection, whereas infectious virus isolation was successful in only 43.33% of attempts.
Why the study?
Rapid and reliable identification of BVDV is essential for disease control programs and confirming acute clinical cases, requiring evaluation of detection methods across laboratories.
Comparison
ERNS-based ELISA vs diverse real-time RT-PCR assays vs virus isolation
Design
International interlaboratory proficiency trial
Authors
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Reinforces PI calf testing in BVDV programs; leaves open optimal rapid diagnostics for field use.
RT-qPCR methods or ERNS antigen ELISAs should be preferentially used for BVDV identification in clinical cases or disease control.
Wernike et al. (2024) studied Bovine viral diarrhea virus (BVDV) (n=40). BVDV detection methods (ERNS antigen ELISA, RT-qPCR, virus isolation) was evaluated on Successful isolation of infectious virus. RT-qPCR and ERNS antigen ELISAs provided reliable BVDV detection, whereas infectious virus isolation was successful in only 43.33% of attempts.
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