Dual Culture of Solanum tuberosum and Synchytrium endobioticum (Pathotype 2)

Synchytrium endobioticum, the infective agent for wart disease of potato, is an obligate pathogen establishing itself successfully on members of the Solanaceae. Its obligate nature and the absence of a monoxenic culture system have limited the level of biological knowledge attainable. We describe a monoxenic culture system using micropropagated potato plantlets. The pathogen was transferred successfully to a second set of plantlets in vitro which yielded resting spores, thus satisfying Koch's postulates for this disease.