Non-Woven Electrostatic Media for Chromatographic Separation of Biological Particles

Nano alumina fibers, 2 nm in diameter and approximately 0.25 µm long are electroadhesively grafted to a microglass fiber. A non-woven media is formed by conventional wet-laid paper making technology. The media has a high affinity for virus, DNA/RNA, proteins, endotoxins, and antigens. A model was developed using data from adsorption of 30 nm latex spheres and was verified with MS2 coliphage. The media is capable of separating equal size α3 and MS2 viruses. The process may be used for concentration and separation of biological particles, at high rates of flow and at pressures less than ∼1 bar over ambient.