Enhanced chiral separation of dansylated amino acids with cyclodextrin‐dextran polymer network by capillary electrophoresis

A method for the chiral separation of dansyl-DL-amino acid mixtures using a dextran (M(r) 2,000,000) polymer network containing beta-cyclodextrin (beta-CD) in HPCE was developed. Mixtures of up to seven amino acids could be baseline resolved by this method under neutral pH conditions. Resolution was dependent on the dextran concentration in the polymer network. Temperature effects on the chiral separation were studied. Optimal efficiency and resolution of DL-enantiomeric pairs of amino acid samples were obtained at 25 degrees C. The resolution of different amino acids used in this work decreased with an increase in temperature.