During sterilization of a culture medium in the presence of NaNO 2 an inhibitor for the growth of Clostridium was observed. The possibility this might be certain volatile N‐nitroso compounds was investigated using CC‐MS. The minimum inhibitory concentration (MIC) of NO 2 ‐ in the medium autoclaved with NO; was 40 ppm; in medium to which NO; was added aseptically, the MIC was 640 ppm. Chromatograms showed peaks with retention times similar to N‐nitroso compounds; however, structure confirmation with MS showed none contained a nitrosoamine and additional experiments confirmed nitrosamines had no inhibitory effects to growth of Clostridiu.
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Wasserman et al. (1972) studied this question.
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