Key result
The developed indirect ELISA using recombinant σC protein successfully detected N-ARV antibodies with a 92.8% positive rate in 363 clinical serum samples from diseased chickens.
Why the study?
A novel avian orthoreovirus caused poor chicken growth and economic losses, but effective methods for detecting antibody levels were lacking.
Does the developed indirect ELISA accurately detect antibodies against novel chicken orthoreovirus in clinical serum samples compared to a commercial kit?
Does the developed indirect ELISA accurately detect antibodies against novel chicken orthoreovirus in clinical serum samples compared to a commercial kit?
The newly developed indirect ELISA using recombinant σC protein provides a sensitive, specific, and repeatable method for detecting novel avian orthoreovirus antibodies in chickens, outperforming a standard commercial kit.
Answers are grounded in this paper and cite their sources.
May enable N-ARV antibody detection in poultry; leaves open prospective validation before diagnostic adoption.
A novel avian orthoreovirus (N-ARV) variant characterized with obvious arthritis and synovial inflammation, was isolated from Shandong, China in May 2016. It caused chicken poor growth and enormous economic losses to the poultry industry of China. However, there are few effective methods for detecting the antibody levels of N-ARV. In this study, a viral structural protein σC was expressed using the prokaryotic expression vector pET32a (+). The target protein was obtained by inducing for 6 hours at an IPTG concentration of 0.6mM. The optimal dilution of the coating antigen and serum antibody were determined to be 1000 fold and 10 fold respectively. A specificity test showed that there was no positive reactivity between N-ARV and other pathogens, and when the positive serum was diluted 100 times detection results were still checkable. The repeatability of this method was determined by the inter assay and intra assay tests with variability ranging from 4.85% to 7.93%. In conclusion, this indirect enzyme linked immunosorbent assay (ELISA) will be useful for large-scale serological surveys and monitoring antibody levels in N-ARV infection.
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Liu et al. (2022) studied Novel avian orthoreovirus (N-ARV) infection (n=453). Indirect ELISA using recombinant σC protein vs. Commercial IDEXX Avian reovirus antibody detection kit was evaluated on Positive detection rate of N-ARV antibodies in clinical samples. The developed indirect ELISA using recombinant σC protein successfully detected N-ARV antibodies with a 92.8% positive rate in 363 clinical serum samples from diseased chickens.
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