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Methodological study demonstrates accurate measurement of digesta free fatty acids in calves, enabling comprehensive analysis of dietary lipid digestion.
A simple method is described suitable for the measurement of free fatty acids, from C4 to C18, produced by lipolysis of dietary fat in the calf. The method involves extraction of samples of diets and digesta with diethyl ether, and absorption of free fatty acids on to an ion exchange resin. After elution and conversion to methyl esters the fatty acids are determined by gas chromatography. By combining the values obtained with those for esterified fatty acids, estimated by an established procedure a complete analysis of the lipid classes in digesta can be obtained.
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J. D. Edwards‐Webb (1975) studied this question.
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