What question did this study set out to answer?

To develop and optimize a rapid, reliable, and precise capillary gas chromatographic method for routine quantification of short- and long-chain free fatty acids in milk and cheese.

February 1, 1990Journal of High Resolution Chromatography

Determination of free fatty acids in milk and cheese procedures for extraction, clean up, and capillary gas chromatographic analysis

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Authors

CJCatrienus de JongWageningen University & Research HBH. T. BadingsNederlands Instituut Voor Zuivel Oonderzoek

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Implication

Analytical study demonstrates rapid quantification of free fatty acids in dairy products, highlighting high extraction recovery and repeatability.

Key Points

To develop and optimize a rapid, reliable, and precise capillary gas chromatographic method for routine quantification of short- and long-chain free fatty acids in milk and cheese.
Optimized lipid extraction using ether-heptane and 2.5 M sulfuric acid, combined with sodium sulfate grinding for cheese and ethanol addition for milk, alongside internal standards for lower free fatty acids (C2:0–C8:0).
Employed an aminopropyl column for isolating free fatty acids from lipid extracts.
Separated underivatized free fatty acids directly using capillary gas chromatography within 40 minutes for chain lengths C2:0 to C20:0.
Ether-heptane extraction recovered 95% to 100% of free fatty acids from cheese and milk (≤ C8:0).
Aminopropyl column isolation achieved 98% to 100% recovery with substantial time savings.
Major free fatty acids (C2:0–C18:3) were quantified within 40 minutes with high repeatability, achieving a relative standard deviation under 2%.

Cite This Study

Jong et al. (1990) studied this question.

synapsesocial.com/papers/6a128ebe1d9aa3bb4e3460d2 https://doi.org/10.1002/jhrc.1240130204

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Implication

Analytical study demonstrates rapid quantification of free fatty acids in dairy products, highlighting high extraction recovery and repeatability.

Determination of free fatty acids in milk and cheese procedures for extraction, clean up, and capillary gas chromatographic analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

Determination of free fatty acids in milk and cheese procedures for extraction, clean up, and capillary gas chromatographic analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

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