Differential RNA editing of mitochondrial atp1 gene in Catharanthus roseus tissues

RNA editing is a posttranscriptional modification.This modification is executed by insertion, deletion or nucleotide substitution.ATP synthase subunit1 (ATP1) gene is encoded to the alpha subunit of complex V which is indispensable for generating mitochondrial ATP.RNA editing in this gene was investigated in many plants like Arabidopsis and Gossypium hirsutum.In this study, five RNA editing sites were in ATP1 gene transcripts in different Catharanthus roseus tissues using high throughput sequencing.Editing sites (C1039, C1178, C1292, C1415, and C1490) were identified from C to T substitution in C. roseus tissues.Results indicate that RNA editing in ATP1 varies across C. roseus tissues.Three edits of ATP1 gene transcripts (C1039, C1292 and C1415) were detected in all C. roseus tissues.The most frequent number of edits appeared in flower and leaf ATP1 tissues of C. roseus compared to other tissues.A C1178 edit of ATP1 transcript was only observed in flower, leaf and hairy root of C. roseus, whereas it was absent in root, stem and seedling.Furthermore, C1490 site of this gene was recognized in all C. roseus tissues except in hairy root tissue.Differential editing of ATP1 gene transcript might control the rate of ATP production.