Lecithinase A catalyzes the removal of a fatty acid from lecithin or the cephalins to yield lyso- lecithin or the lysocephalins which are particu- larly noted for their lytic effect on red blood cells (1).Hanahan (2) studied the action of the enzyme in extracts of pancreatin on lecithin in diethyl ether.This method was used by Hanahan, Rodbell and Turner (3) and improved by Long and Penny (4) in studies of the action of the en- zyme in venom on natural and synthetic phosphoglycerides.Epstein and Shapiro (5) and Rimon and Shapiro (6) measured the enzyme from rat intestinal mucosa and ox pancreas in phosphate buffer.
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Vogel et al. (1960) studied this question.