Structural study reveals how KlenTaq DNA polymerase processes consecutive C5-modified nucleotides, highlighting mechanisms governing enzyme interactions with synthetic genetic substrates.
Functional nucleotides are important in many cutting-edge biomolecular techniques. Often several modified nucleotides have to be incorporated consecutively. This structural study of KlenTaq DNA polymerase, a truncated form of Thermus aquaticus DNA polymerase, gives first insights how multiple modifications are processed by a DNA polymerase and, therefore, contribute to the understanding of these enzymes in their interplay with artificial substrates.
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Obeid et al. (2013) studied this question.