The Cytochemical Demonstration of Lysosomal Aryl Sulfatase Activity by Light and Electron Microscopy

mmmcd ismflu sized costec i'soolanmi nmo--coou'st ai oui tog umerve cells its thin Amaldite sectiomus sisowed a rati'sen wo-osk, chiflimse aosd soonmetim's'ses granuumban flumcunescence as coummul000nedl tco time very stnoo'sg green fluorescence so-en's it's paraflimo sect icon's (8 t-o 10 , ) (Doohlstn#{246}n's and Fouxe, op.cit.).This stromug fitmono-sceouce nnade it alnsuoost inu'sb)ossii)lo-too observe nuioson changes in amuse coostemmt cub the cell bodies prodtmced by certain piuamnm'sacobogic stut)stau'sces, e.g., MAO-inhihitoums.Tisios Amaldite sectiooms, ti'scro-bore, provicle os ossoore seomsitive i)asis for stmci's expeninmen'st-s.Iou coosclumsiooum, tiuin so-ctium'ss of Araldite-enm-bedded tissumes mmsoov boo-a voolunable complcnmueuut to the usual panafflo's embedding, otTo-ring nmeaoss of overcoming (he gap between electron and flumoresco-nec nm'sicmooscopy, and providing a moore exact and sensitive basis for statistical aomabyses aumd for estin'sations of drung-inuduced changes of the ios(raneunonal an'siome levels.