Chemical Constituents and Hemolytic Activity of Macrotyloma uniflorum L.

In the present investigation, attempt was taken to chromatographic separation and isolation of dichloromethane extract of aerial parts of Macrotyloma uniflorum Linn afforded a total of two compounds, methyl ester of hexadecanoic- and ethyl ester of hexadecanoic acid mixture (I), and n-hexadecanoic acid (II). The structures of these compounds were elucidated by spectroscopic analyses, including UV, IR, 1 H-NMR, 13 C-NMR and mass spectroscopy. In addition, the fractionated crude extracts dichloromethane (CH 2 Cl 2 ), ethyl acetate (EtOAc), 1-butanol (1-BuOH) and aqueous (H 2 O) from aerial parts of M. uniflorum were tested for hemolytic assay. Among all tested extracts, only the extract of 1-butanol exhibited the significant hemolytic activity .